• Catalog Number:

  E1A8283A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 70 (T-K-TP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q13541

• Applications:

  IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  4EBP1; EIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Insulin-stimulated EIF-4E binding protein PHAS-I; P/OKCL.6; PHAS-1; PHAS-I

• Specificity Sensitivity:

  4E-BP1 (Phospho-Thr70) antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 70.

• References:

  Pause A., Nature 371:762-767(1994). Gevaert K., Nat. Biotechnol. 21:566-569(2003). Haghighat A., EMBO J. 14:5701-5709(1995).

• All Sites:

  Human: Thr70

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Phospho-Thr70) antibody.