• Catalog Number:

  E1A1043A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human hnRPD around the phosphorylation site of serine 83 (N-S-SP-P-R).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q14103

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  ARE element RNA-binding protein AUF1, AU-rich element RNA-binding protein 1, AUF1, HNRPD, Heterogeneous nuclear ribonucleoprotein D, Heterogeneous nuclear ribonucleoprotein D0, ROD

• Specificity Sensitivity:

  hnRPD (Phospho-Ser83) antibody detects endogenous levels of hnRPD only when phosphorylated at serine 83.

• References:

  Kajita Y., J. Biol. Chem. 270:22167-22175(1995). Dempsey L.A., Genomics 49:378-384(1998). Tolnay M., Biochem. J. 338:417-425(1999). Grosset C., Cell 103:29-40(2000).

• All Sites:

  Human: Ser52; Mouse: Ser52

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using hnRPD (Phospho-Ser83) antibody. Western blot analysis of extracts from HuvEc cells (lane 1) and HT29 cells (lane 2), using hnRPD (Phospho-Ser83) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using hnRPD (Phospho-Ser83) antibody.