• Catalog Number:

  E1A8264A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Caveolin 2 around the phosphorylation site of tyrosine 27 (L-E-YP-A-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P51636

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  CAV2; Caveolin-2

• Specificity Sensitivity:

  Caveolin 2 (Phospho-Tyr27) antibody detects endogenous levels of Caveolin 2 only when phosphorylated at tyrosine 27.

• References:

  Scherer P.E., Proc. Natl. Acad. Sci. U.S.A. 93:131-135(1996). Scherer P.E., J. Biol. Chem. 272:29337-29346(1997). Engelman J.A., FEBS Lett. 448:221-230(1999).

• All Sites:

  Human: Tyr27

Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 5mins), using Caveolin 2 (Phospho-Tyr27) antibody.