• Catalog Number:

  E012020-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FOXO3A around the phosphorylation site of serine 253 (A-V-SP-M-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O43524

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  FOXO3A (phospho-Ser253) antibody detects endogenous levels of FOXO3A only when phosphorylated at serine 253.

• References:

  Li M, Chiu JF, Mossman BT, Fukagawa NK (2006) D J Biol Chem 281, 40429-39 Essafi A, et al. (2005) Oncogene 24, 2317-29 Seoane, J. et al. (2004) Cell 117, 211-223. Brunet A, et al. (2001) Mol Cell Biol 21, 952-65 Brunet A, et al. (1999) Cell 96, 857-68

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FOXO3A (phospho- Ser253) antibody (E012020).

Western blot analysis of extracts from NIH/3T3 cells treated with using Serum. Left: Using FOXO3A (phospho-Ser253) antibody (E012020). Right: Using the same antibody preincubated with synthesized peptide.