• Catalog Number:

  E1A8251A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human VE-Cadherin around the phosphorylation site of tyrosine 731 (H-I-YP-G-Y).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%)

• SwissProtNo:

  P33151

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  7B4 antigen; CADH5; Cadherin-5; CD144 antigen; Vascular endothelial-cadherin precursor; VE-cadherin

• Specificity Sensitivity:

  VE-Cadherin (Phospho-Tyr731) antibody detects endogenous levels of VE-Cadherin only when phosphorylated at tyrosine 731.

• References:

  Breviario F., Arterioscler. Thromb. Vasc. Biol. 15:1229-1239(1995). Ali J., Microcirculation 4:267-277(1997). Shimoyama Y., Biochem. J. 349:159-167(2000).

• All Sites:

  Human: Tyr731; Mouse: Tyr731

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using VE-Cadherin (Phospho-Tyr731) antibody. Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3mM, 40mins), using VE-Cadherin (Phospho-Tyr731) antibody.