Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human HP1α around the phosphorylation site of serine 92 (R-K-SP-N-F).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human (Identities = 100%, Positives = 100%)
SwissProtNo:
P45973
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Other Names:
antigen p25; chromobox protein homolog 5; heterochromatin protein 1 alpha; HP1 alpha; HP1A
Specificity Sensitivity:
HP1α (Ab-92) antibody detects endogenous levels of total HP1α protein.
References:
Saunders W.S., J. Cell Sci. 104:573-582(1993).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Ye Q., J. Biol. Chem. 271:14653-14656(1996).
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Peptide - +
Immunohistochemistry analysis of paraffin-embedded human braina tissue using HP1α (Ab-92) antibody.
Western blot analysis of extracts from Jurkat cells, treated with insulin (0.01U/ml, 15mins), using HP1α (Ab-92) antibody.