• Catalog Number:

  E1A8216A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human BLNK around the phosphorylation site of tyrosine 84 (E-M-YP-V-M).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q8WV28

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  B-cell linker protein; LY57; SLP-65; SLP65

• Specificity Sensitivity:

  BLNK (Phospho-Tyr84) antibody detects endogenous levels of BLNK only when phosphorylated at tyrosine 84.

• References:

  Fu C., Immunity 9:93-103(1998). Minegishi Y., Science 286:1954-1957(1999). The MGC Project Team; Genome Res. 14-2121-2127(2004).

• All Sites:

  Human: Tyr84; Mouse: Tyr84; Rat: Tyr84

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using BLNK (Phospho-Tyr84) antibody. Western blot analysis of extracts from K562 cells, treated with starved (24hours), using BLNK (Phospho-Tyr84) antibody.