Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human IGF1R around the phosphorylation site of tyrosine 980 (P-E-YP-F-S ).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P08069
Applications:
IHC: 1:50~1:100
Specificity Sensitivity:
IGF1R (Phospho-Tyr980) antibody detects endogenous levels of IGF1R only when phosphorylated at tyrosine 980.
References:
Craparo A., et al. (1995) J. Biol. Chem. 270:15639-15643.
Adams, T.E. et al. (2000) Cell. Mol. Life Sci. 57, 1050-1093.
Baserga, R. et al. (2000) Oncogene 19, 5574-5581.
Scheidegger, K.J. et al. (2000) J. Biol. Chem. 275, 38921-38928.
Baserga, R. et al. (1999) Exp. Cell Res. 253, 1-6.
White, M.F. et al. (1985) J. Biol. Chem. 260, 9470-9478.
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using IGF1R (Phospho-Tyr980) Antibody. (E012089).