• Catalog Number:

  E1A8215A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human LCK around the phosphorylation site of serine 59 (P-A-SP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 92%);Rat (Identities = 92%, Positives = 92%)

• SwissProtNo:

  P06239

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  EC 2.7.10.2; kinase Lck; LCK; LSK; LSK-T; lymphocyte-specific protein tyrosine kinase; P56-LCK; T cell-specific protein-tyrosine kinase

• Specificity Sensitivity:

  LCK (Phospho-Ser59) antibody detects endogenous levels of LCK only when phosphorylated at serine 59.

• References:

  Koga Y., Eur. J. Immunol. 16:1643-1646(1986). Perlmutter R.M., J. Cell. Biochem. 38:117-126(1988). Rouer E., Gene 84:105-113(1989). ----------------------------------------------------------------------------

• All Sites:

  Human: Ser59; Mouse: Ser59; Rat: Ser59

Lane 1: The extracts from Jurkat cells treated with IFN (2500U/ML, 30mins) using LCK (Phospho-Ser59) antibody antibody Lane 2: The extracts from Jurkat cells treated with IFN (2500U/ML, 30mins) plus phosphopeptide using LCK (Phospho-Ser59) antibody antibody