• Catalog Number:

  E1A8206A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human BIM around the phosphorylation site of serine 59 (H-G-SP-P-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O43521

• Applications:

  IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  Bcl-2 related ovarian death protein; BCL2 interacting mediator of cell death; Bcl2 interacting mediator of cell death; BCL2-like protein 11; Bcl2-like protein 11; BCL2L11; BOD

• Specificity Sensitivity:

  BIM (Phospho-Ser59) antibody detects endogenous levels of BIM only when phosphorylated at serine 59.

• References:

  O'Connor L., EMBO J. 17:384-395(1998). Mami U., FEBS Lett. 509:135-141(2001). Liu J.-W., Cancer Res. 62:2976-2981(2002).

• All Sites:

  Human: Ser59; Mouse: Ser55; Rat: Ser55

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human brain tissue, using BIM (Phospho-Ser59) antibody.