• Catalog Number:

  E1A7164A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p100/p52 around the phosphorylation site of Serine 865.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q00653

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 IP: Various Dilution

• Specificity Sensitivity:

  NF-κB p100/p52 (Phospho-Ser865) Antibody detects endogenous levels of NF-κB p100/p52 only when phosphorylated at Serine 865.

• References:

  Qu Z, et al. (2004) J Biol Chem. 279(43): 44563-44572. Xiao G, et al. (2001) Mol Cell. 7(2): 401-409. Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20.

Immunohistochemical analysis of paraffin- embedded breast carcinoma. Left: Using NF-κB p100/p52 (Phospho-Ser865) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.

Western blot analysis of extracts from ovary cancer cells, using NF-κB p100/p52 (Phospho-Ser865) Antibody. Line1: Untreated; Line2: Treated with synthesized phosphopeptide.