• Catalog Number:

  E1A8177A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human C-RAF around the phosphorylation site of threonine 269 (S-T-TP-L-P).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 92%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P04049

• Applications:

  IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  C-RAF; C-Raf; CRAF; EC 2.7.11.1; kinase Raf1; Protein kinase raf 1; RAF proto-oncogene serine/threonine-protein kinase; Raf proto-oncogene serine/threonine-protein kinase; RAF-1; Raf-1; RAF1; Similar to murine leukemia viral (V-raf-1) oncogene homolog 1

• Specificity Sensitivity:

  C-RAF (Phospho-Thr269) antibody detects endogenous levels of C-RAF only when phosphorylated at threonine 269.

• References:

  The MGC Project Team; Genome Res. 14:2121-2127(2004). Olsen J.V., Cell 127:635-648(2006). Mott H.R., Proc. Natl. Acad. Sci. U.S.A. 93:8312-8317(1996).

• All Sites:

  Human: Thr269; Mouse: Thr269; Rat: Thr269

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using C-RAF (Phospho-Thr269) antibody. C-RAF (Phospho-Thr269) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.