• Catalog Number:

  E1A1229A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Proteasome α3 around the phosphorylation site of serine 250 (D-E-SP-D-D).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P25788

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  EC 3.4.25.1; Macropain subunit C8; Multicatalytic endopeptidase complex subunit C8; proteasome component C8; Proteasome subunit alpha type 3; PSA3; PSC8

• Specificity Sensitivity:

  Proteasome α3 (Phospho-Ser250) antibody detects endogenous levels of Proteasome α3 only when phosphorylated at serine 250.

• References:

  Tamura T., Biochim. Biophys. Acta 1089:95-102(1991). Kalnine N., Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases. The MGC Project Team; Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Ser250; Mouse: Ser250; Rat: Ser250

Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 30mins), using Proteasome α3 (Phospho-Ser250) antibody.