• Catalog Number:

  E1A8175A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PLK1 around the phosphorylation site of serine 137 (R-R-SP-L-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P53350

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  Polo-like kinase 1; PLK-1; Serine/threonine-protein kinase 13; STPK13

• Specificity Sensitivity:

  PLK1 (Phospho-Ser137) antibody detects endogenous levels of PLK1 only when phosphorylated at serine 137.

• References:

  Hamanaka R., Cell Growth Differ. 5:249-257(1994). Lake R.J., Mol. Cell. Biol. 13:7793-7801(1993). Golsteyn R.M., J. Cell Sci. 107:1509-1517(1994).

• All Sites:

  Human: Ser137; Mouse: Ser137; Rat: Ser137

Western blot analysis of extracts from Jurkat cells treated with PMA, using PLK1 (Phospho-Ser137) antibody.