Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Rel
around the phosphorylation site of serine 503 (T-S-SP-D-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human
SwissProtNo:
Q04864
Applications:
IF:1:100~1:200
Specificity Sensitivity:
Rel (phospho-Ser503) antibody detects endogenous levels of Rel only when phosphorylated at serine 503.
References:
Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20
Haskill, S. et al. (1991) Cell 65, 1281-9.
Thompson, J.E. et al. (1995) Cell 80, 573-82.
Immunofluorescence staining of methanol-fixed HeLa cells using Rel (phospho-Ser503) antibody (E011020, Red).