• Catalog Number:

  E1A8157A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p70 S6 Kinase β around the phosphorylation site of serine 423 (P-V-SP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 84%, Positives = 92%)

• SwissProtNo:

  Q9UBS0

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  14 beta; 70 kDa ribosomal protein S6 kinase 2; EC 2.7.11.1; kinase p70S6K-beta; KS6B2; p70 ribosomal S6 kinase beta; p70 S6Kbeta; p70-S6KB; p70S6K-beta; Ribosomal protein S6 kinase beta 2; RPS6KB2; S6 kinase-related kinase; S6K-beta 2; S6K2; SRK; STK14B

• Specificity Sensitivity:

  p70 S6 Kinase β (Phospho-Ser423) antibody detects endogenous levels of p70 S6 Kinase β only when phosphorylated at serine 423.

• References:

  Gout I., J. Biol. Chem. 273:30061-30064(1998). Saitoh M., Biochem. Biophys. Res. Commun. 253:470-476(1998). Lee-Fruman K.K., Oncogene 18:5108-5114(1999).

• All Sites:

  Human: Ser423; Mouse: Ser423

Western blot analysis of extracts from K562 cells, treated with EGF (200ng/ml, 5mins), using p70 S6 Kinase β (Phospho-Ser423) antibody.