• Catalog Number:

  E1A8142A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MLK1/2 around the phosphorylation site of threonine 312/266 (A-G-TP-Y-A).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P80192/Q02779

• Applications:

  IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  EC 2.7.11.25; M3K9; mitogen-activated protein kinase kinase kinase 9; mixed-lineage protein kinase 1; PRKE1

• Specificity Sensitivity:

  MLK1/2 (Phospho-Thr312/266) antibody detects endogenous levels of MLK1/2 only when phosphorylated at threonine 312/266.

• References:

  Durkin J.T., Biochemistry 43:16348-16355(2004). The MGC Project Team; Genome Res. 14:2121-2127(2004). Dorow D.S.,Nature 446:153-158(2007).

• All Sites:

  Human: Thr312/266; Mouse: Thr305/266

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using MLK1/2 (Phospho-Thr312/266) antibody. MLK1/2 (Phospho-Thr312/266) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.