Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Tau around the phosphorylation site of threonine 212 (S-R-TP-P-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P10636
Applications:
WB:1:500~1000
Specificity Sensitivity:
Tau (phospho-Thr212) antibody detects endogenous levels of Tau only when phosphorylated at threonine 212.
References:
Magnani E,et al.(2007)EMBO J.26(21):4546-54.
Perez M, et al.(2007)J Neurochem.103(4):1447-60.
Rhein V, et al. (2007)Arch Physiol Biochem.113(3):131-41.
Western blot analysis of extract from mouse brain tissue, using Tau (phospho-Thr212) antibody (E011257, Lane 1 and 2).