• Catalog Number:

  E1A8116A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human INSR around the phosphorylation site of threonine 1375 (I-L-TP-L-P).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%);Rat (Identities = 92%, Positives = 100%)

• SwissProtNo:

  P06213

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  CD220 antigen; EC 2.7.10.1; insulin receptor; IR; kinase InsR

• Specificity Sensitivity:

  INSR (Phospho-Thr1375) antibody detects endogenous levels of INSR only when phosphorylated at threonine 1375.

• References:

  Ebina Y., Cell 40:747-758(1985). Ullrich A., Nature 313:756-761(1985). Seino S., Diabetes 39:123-128(1990).

• All Sites:

  Human: Thr1375; Mouse: Thr1365; Rat: Thr1376

Western blot analysis of extracts from HepG2 cells, using INSR (Phospho-Thr1375) antibody.