• Catalog Number:

  E011501-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human AKT1/AKT2/AKT3 around the phosphorylation site of tyrosine 315/316/312 (P-E-YP-L-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P31749 P31751 Q9Y243

• Applications:

  IHC: 1:50~100

• Specificity Sensitivity:

  AKT1/AKT2/AKT3 (Phospho-Tyr315/316/312) Antibody detects endogenous levels of AKT1/AKT2/AKT3 only when phosphorylated at tyrosine 315/316/312.

• References:

  Baudhuin LM, et al. (2004) FASEB J Feb; 18(2): 341-3. Min YH, et al. (2004) Cancer Res; 64(15): 5225-31. Feng J, et al. (2004) J Biol Chem; 279(34): 35510-7. Ayala G, et al. (2004) Clin Cancer Res; 10(19): 6572-8. Lungu AO, et al. (2004) J Biol Chem; 279(47): 48794-800.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3 (Phospho- Tyr315/316/312) Antibody (E011501).