Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human FLT3 around the phosphorylation site of tyrosine 696 (H-T-YP-Q-N).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
FLT3 (Phospho-Tyr969) antibody detects endogenous levels of FLT3 only when phosphorylated at tyrosine 696.
References:
Small D., Proc. Natl. Acad. Sci. U.S.A. 91:459-463(1994).
Rosnet O., Blood 82:1110-1119(1993).
Rosnet O., Genomics 9:380-385(1991).
All Sites:
Human: Tyr969
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using FLT3 (Phospho-Tyr969) antibody.
Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3mM, 40mins), using FLT3 (Phospho-Tyr969) antibody.