• Catalog Number:

  E1A1221A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Nibrin around the phosphorylation site of serine 278 (T-N-SP-Q-T).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%);

• SwissProtNo:

  O60934

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  Cell cycle regulatory protein P95; NBN; NBS; NIBRIN; Nijmegen breakage syndrome 1 (nibrin); NIJMEGEN BREAKAGE syndrome protein 1; p95-NBS1

• Specificity Sensitivity:

  Nibrin (Phospho-Ser278) antibody detects endogenous levels of Nibrin only when phosphorylated at serine 278.

• References:

  Varon R., Cell 93:467-476(1998). Carney J.P., Cell 93:477-486(1998). Matsuura S., Nat. Genet. 19:179-181(1998).

• All Sites:

  Human: Ser278

P-peptide - + Western blot analysis of extracts from HUVEC cells, treated with Forskolin (40nM, 30mins), using Nibrin (Phospho-Ser278) antibody. Immunofluorescence analysis of NIH/3T3 cells, using Nibrin (Phospho-Ser278) antibody.

Nibrin (Phospho-Ser278) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.