• Catalog Number:

  E1A1242B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human Vimentin around the phosphorylation site of serine 83 (Q-D-SP-V-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P08670

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  VIME, vimentin

• Specificity Sensitivity:

  Vimentin (Ab-83) antibody detects endogenous levels of total Vimentin protein.

• References:

  Ferrari S., Mol. Cell. Biol. 6:3614-3620(1986). Hartmann T.B., Br. J. Dermatol. 150:252-258(2004). Ota T., Nat. Genet. 36:40-45(2004).

Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using Vimentin (Ab-83) antibody.