Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Trk A around the phosphorylation site of tyrosine 701 (I-L-YP-R-K).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Trk A (Phospho-Tyr701) antibody detects endogenous levels of Trk A only when phosphorylated at tyrosine 701.
References:
Martin-Zanca D., Mol. Cell. Biol. 9:24-33(1989).
Shelton D.L., J. Neurosci. 15:477-491(1995).
Indo Y., Jpn. J. Hum. Genet. 42:343-351(1997).
All Sites:
Human: Tyr701; Mouse: Tyr704; Rat: Tyr704
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Trk A (Phospho-Tyr701) antibody.
Western blot analysis of extracts from mouse brain cells, using Trk A (Phospho-Tyr701) antibody.