• Catalog Number:

  E011157-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FKHRL1 around the phosphorylation site of serine 253 (A-V-SP-M-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O43524

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  FKHRL1 (phospho-Ser253) antibody detects endogenous levels of FKHRL1 only when phosphorylated at serine 253.

• References:

  Smith WW, et al. (2005) J Cell Biol; 169(2): 331-9. Essafi A, et al. (2005) Oncogene; 24(14): 2317-29. Brunet A, et al. (2001)Mol Cell Biol; 21(3): 952-65. Brunet A, et al. (1999) Cell; 96(6): 857-68.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHRL1 (phospho-Ser253) antibody (E011157).

Western blot analysis of extracts from NIH/3T3 cells using FKHRL1 (Ab-253) antibody (E021171, Lane 1 and 2) and FKHRL1 (phospho-Ser253) antibody (E011157, Lane 3 and 4).