• Catalog Number:

  E1A8059A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Retinoblastoma around the phosphorylation site of serine 608 (Y-L-SP-P-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 93%, Positives = 100%);Rat (Identities = 93%, Positives = 100%)

• SwissProtNo:

  P06400

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  P105-RB; PP105; PP110; RB-1; RB1; retinoblastoma gene; Retinoblastoma-associated protein

• Specificity Sensitivity:

  Retinoblastoma (Phospho-Ser608) antibody detects endogenous levels of Retinoblastoma only when phosphorylated at serine 608.

• References:

  Lee W.-H., Nature 329:642-645(1987). Lee W.-H., Science 235:1394-1399(1987). Friend S.H., Proc. Natl. Acad. Sci. U.S.A. 84:9059-9063(1987). - ---------------------------------------------------------------------------------------------------------------------------------------------------------------

• All Sites:

  Human: Ser608; Mouse: Ser601; Rat: Ser579

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Retinoblastoma (Phospho-Ser608) antibody. Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using Retinoblastoma (Phospho-Ser608) antibody.