• Catalog Number:

  E1A8053A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of serine 366 (A-H-SP-S-H).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P04637

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  Antigen NY-CO-13; Cellular tumor antigen p53; Phosphoprotein p53; TP53; tumor protein p53; Tumor suppressor p53

• Specificity Sensitivity:

  p53 (Phospho-Ser366) antibody detects endogenous levels of p53 only when phosphorylated at serine 366.

• References:

  Zakut-Houri R., EMBO J. 4:1251-1255(1985). Lamb P., Mol. Cell. Biol. 6:1379-1385(1986). Harlow E., Mol. Cell. Biol. 5:1601-1610(1985).

• All Sites:

  Human: Ser366

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using p53 (Phospho-Ser366) antibody. Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using p53 (Phospho-Ser366) antibody.