• Catalog Number:

  E011293-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MARCKS around the phosphorylation site of serine 158 (R-F-SP-F-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P29966

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  MARCKS (phospho-Ser158) antibody detects endogenous levels of MARCKS only when phosphorylated at serine 158.

• References:

  Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102(35): 12407-12412 Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280(3): 605-609 Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359(2): 151-159

Western blot analysis of extract from starved NIH/3T3 cells, using MARCKS (Ab-158) antibody (E021285, Lane 1 and 2) and MARCKS (phospho-Ser158) antibody (E011293, Lane 3 and 4).