Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human AKT1/3 around the phosphorylation site of tyrosine 437/434 (T-R-YP-F-D).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
AKT1/3 (Ab-437/434) antibody detects endogenous levels of total AKT1/3 protein.
References:
Jones P.F., Proc. Natl. Acad. Sci. U.S.A. 88:4171-4175(1991).
Matsubara A., Diabetologia 44:910-913(2001).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Western blot analysis of extracts from A549 cells, using AKT1/3 (Ab-437/434) antibody.