• Catalog Number:

  E1A8134A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MAP3K8 around the phosphorylation site of serine 400 (C-Q-SP-L-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P41279

• Applications:

  IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  C-COT; cancer Osaka thyroid oncogene; COT proto-oncogene serine/threonine-protein kinase; EC 2.7.11.25; kinase Cot; M3K8; MAP3K8; mitogen-activated protein kinase kinase kinase 8; TPL-2; Tpl2; TPL2; tumor progression locus 2

• Specificity Sensitivity:

  MAP3K8 (Phospho-Ser400) antibody detects endogenous levels of MAP3K8 only when phosphorylated at serine 400.

• References:

  Miyoshi J., Mol. Cell. Biol. 11:4088-4096(1991). Aoki M., J. Biol. Chem. 268:22723-22732(1993). Chan A.M.,Oncogene 8:1329-1333(1993).

• All Sites:

  Human: Ser400; Mouse: Ser400; Rat: Ser400

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human brain tissue using MAP3K8 (Phospho-Ser400) antibody.