• Catalog Number:

  E1A8023A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of serine 695 (T-P-SP-G-E).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P00533

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB- ; EGFR; ERBB1

• Specificity Sensitivity:

  EGFR (Phospho-Ser695) antibody detects endogenous levels of EGFR only when phosphorylated at serine 695.

• References:

  Ullrich A., Nature 309:418-425(1984). Ilekis J.V., Mol. Reprod. Dev. 41:149-156(1995). Reiter J.L., Nucleic Acids Res. 24:4050-4056(1996).

• All Sites:

  Human: Ser695; Mouse: Ser697; Rat: Ser696

Western blot analysis of extracts from 293 cells and K562 cells treated with EGF, using EGFR (Phospho-Ser695) antibody.