Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Tau around the phosphorylation site of Threonine 181(P-K-TP-P-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P10636
Applications:
WB: 1:500~1:1000 ELISA: 1:20000
Specificity Sensitivity:
Tau (Phospho-Thr181) Antibody detects endogenous levels of Tau only when phosphorylated at Threonine 181.
References:
Puig B, et al.(2005) Acta Neuropathol (Berl). 110(3):261-268.
Pérez M, et al.(2005) Neuroscience. 130(2): 339-347.
Fellgiebel A, et al.(2004) Biol Psychiatry. 56(4): 279-283.
del C Alonso A, et al.(2004) J Biol Chem. 279(33): 34873-34881.
Western blot analysis of extracts from mouse brain tissue using Tau (Ab-181) Antibody and Tau (Phospho-Thr181) Antibody.