• Catalog Number:

  E1A1246A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Werner Syndrome Helicase around the phosphorylation site of serine 1141 (S-S-SP-Q-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  Q14191

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  EC 3.6.1.-, RECQ3, RECQL2, RECQL3, Werner Syndrome helicase

• Specificity Sensitivity:

  Werner Syndrome Helicase (Phospho-Ser1141) antibody detects endogenous levels of Werner Syndrome Helicase only when phosphorylated at serine 1141.

• References:

  Yu C.-E., Science 272:258-262(1996). Marciniak R.A., Proc. Natl. Acad. Sci. U.S.A. 95:6887-6892(1998). Kusano K., Genetics 151:1027-1039(1999).

• All Sites:

  Human: Ser1141

Western blot analysis of extracts from K562 cells, treated with etoposide (25uM, 24hours), using Werner Syndrome Helicase (Phospho-Ser1141) antibody.