• Catalog Number:

  E1A7243A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Tau around the phosphorylation site of serine 422 (V-D-SP-P-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P10636

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 ELISA: 1:10000

• Alter Name:

  MAPT, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau

• Specificity Sensitivity:

  Tau (phospho-Ser422) antibody detects endogenous levels of Tau only when phosphorylated at serine 422.

• References:

  Mario Binelli, Biol Reprod, Feb 2001; 64: 654. Jens Leipziger, Am J Physiol Renal Physiol, Nov 2000; 279: 919. Yuuki Kawamura, Eur. J. Biochem., May 2001; 268: 3036. Xiaoying Koh, Biophys. J., Mar 2006; 90: 1999 - 2014.

• All Sites:

  Human: Ser422; Mouse: Ser713; Rat: Ser732

P-peptide - + Immunohistochemical analysis of paraffin-embedded human brain tissue using Tau (phospho-Ser422) antibody.

Western blot analysis of extracts from mouse brain, using Tau (phospho-Ser422) antibody.