• Catalog Number:

  E1A7235A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human α-Synuclein around the phosphorylation site of tyrosine 133 (E-G-YP-Q-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P37840

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Alter Name:

  NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, SYN, SYUA, alpha-synuclein, synuclein alpha

• Specificity Sensitivity:

  α-Synuclein (phospho-Tyr133) antibody detects endogenous levels of α-Synuclein only when phosphorylated at tyrosine 133.

• References:

  Yangfu Jiang, Cancer Res., Jul 2004; 64: 4539 - 4546. Alessandro Negro, FASEB J, Dec 2001; 10.1096. ALESSANDRA RECCHIA, FASEB J, Apr 2004; 18: 617 - 626. Christopher E. Ellis, J. Biol. Chem., Feb 2001; 276: 3879 - 3884.

• All Sites:

  Human: Tyr133; Mouse: Tyr133; Rat: Tyr133

Western blot analysis of extracts from 293 cells treated with Etoposide (25μM, 60mins), using α-Synuclein (phospho-Tyr133) antibody.