• Catalog Number:

  E1A7229A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human STAT6 around the phosphorylation site of Tyrosine 641(R-G-YP-V-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P42226

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Specificity Sensitivity:

  STAT6(Phospho-Tyr641) Antibody detects endogenous levels of STAT6 only when phosphorylated at Tyrosine 641.

• References:

  Nelms K, et al. (1999) Annu Rev Immunol. 17:701-738. Malabarba M G, et al. (1996) Biochem. J. 319:865-872. Hou J, et al. (1994) Science. 265:1701-1706. Quelle F W, et al. (1995) Mol Cell Biol. 15: 3336-3343.

Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using STAT6 (Phospho-Tyr641) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.

Western blot analysis using STAT6 (Phospho- Tyr641) Antibody: Line1: The extracts from HeLa cells; Line2: The extracts from HeLa cells treated with IL-4.