Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human
ABL1/2 around the phosphorylation site of tyrosine 393/429 (D-T-YP-T-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
P00519 P42684
Applications:
IHC: 1:50~100
Specificity Sensitivity:
ABL1/2 (phospho-Tyr393/429) Antibody detects endogenous levels of ABL1/2 only when
phosphorylated at tyrosine393/429.
References:
Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650.
Danial, N.N. et al. (2000) Oncogene 19, 2523-2531.
Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637.
Pluk, H. et al. (2002) Cell 108, 247-259.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/2 (phospho-Tyr393/429) Antibody (E011530).