Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human STAT6 around the phosphorylation site of threonine 645 (P-A-TP-I-K).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human
SwissProtNo:
P42226
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:10000 IP: Various Dilution
Specificity Sensitivity:
STAT6 (phospho-Thr645) antibody detects endogenous levels of STAT6 only when phosphorylated at threonine 645.
References:
Nelms K, et al. (1999) Annu Rev Immunol. 17:701-738.
Malabarba M G, et al. (1996) Biochem. J. 319:865-872.
Hou J, et al. (1994) Science. 265:1701-1706.
Quelle F W, et al. (1995) Mol Cell Biol. 15: 3336-3343.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT6 (phospho-thr645) antibody.