• Catalog Number:

  E1A7068A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Elk-1 around the phosphorylation site of Serine 383.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P19419

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 IP: Various Dilution

• Specificity Sensitivity:

  Elk-1 (Phospho-Ser383) Antibody detects endogenous levels of Elk-1 only when phosphorylated at Serine 383.

• References:

  Xing J, et al. (1996) Science. 273(5277): 959-963. Janknecht R, et al. (1993) EMBO J. 12(13): 5097-5104. Marais R, et al. (1993) Cell 73: 381-393. Kortenjann M, et al. (1994) Mol Cell Biol. 14: 4815-4824. Cavigelli M, et al. (1995) EMBO J. 14: 5957-5964.

Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using Elk-1 (Phospho- Ser383) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.

Western blot analysis of extracts using Elk-1 (Phospho-Ser383) Antibody.Line1: The extracts from HeLa cells untreated; Line2: The extracts from HeLa cells treated with UV.