Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human STAT4 around the phosphorylation site of tyrosine 693 (K-G-YP-V-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q14765
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:5000 IP: Various Dilution
Specificity Sensitivity:
STAT4 (phospho-Tyr693) antibody detects endogenous levels of STAT4 only when phosphorylated at tyrosine 693.
References:
Strausberg R L, et al. (2002) Proc Natl Acad Sci U S A. 99(26):16899-16903.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT4 (phospho-Tyr693) antibody.
Western blot analysis of extracts from HeLa cells using STAT4 (phospho-Tyr693) antibody.