• Catalog Number:

  E1A7222A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human STAT1 around the phosphorylation site of tyrosine 701 (T-G-YP-I-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P42224

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 IP: Various Dilution

• Specificity Sensitivity:

  STAT1 (phospho-Tyr701) antibody detects endogenous levels of STAT1 only when phosphorylated at tyrosine 701.

• References:

  Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120. Durbin J E, et al. (1996) Cell. 84: 443-450. Demoulin J, B. et al. (1999) J Biol Chem. 274: 25855-258. Ihle J N, et al. (1994) Trends Biochem Sci. 19: 222-227.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT1 (phospho-Tyr701) antibody.

Western blot analysis of extracts from MCF7 cells using STAT1 (phospho-Tyr701) antibody.