Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Tau around the phosphorylation site of Serine 214.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P10636
Applications:
WB: 1:500~1:1000 ELISA: 1:5000
Specificity Sensitivity:
Tau (Phospho-Ser214) Antibody detects endogenous levels of Tau only when phosphorylated at Serine 214.
References:
Smet C, et al. (2005) FEBS Lett; 579(19): 4159-64.
Puig B, et al. (2005) Acta Neuropathol (Berl).
Gvtz J, et al. (2001) Science; 293(5534): 1491-5.
Illenberger S, et al. (1998) Mol Biol Cell; 9(6): 1495-512.
Western blot analysis of extracts using Tau (Ab-214) Antibody. Line1: The extracts from HeLa cells; Line2: The extracts from EKVX cells.