• Catalog Number:

  E1A7218A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SP1 around the phosphorylation site of threonine 739 (T-A-TP-P-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P08047

• Applications:

  WB: 1:500~1:3000 ELISA: 1:5000

• Other Names:

  Transcription factor SP1; transcription factor Sp1; TSFP1

• Specificity Sensitivity:

  SP1 (Phospho-Thr739) antibody detects endogenous levels of SP1 only when phosphorylated at threonine 739.

• References:

  The MGC Project Team; Genome Res. 14:2121-2127(2004). Haggart M.H., Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases. Takahara T., J. Biol. Chem. 275:38067-38072(2000).

• All Sites:

  Human: Thr739; Mouse: Thr738; Rat: Thr740

Western blot analysis of extracts from COS cells, treated with serum (20%, 15mins), using SP1 (Phospho-Thr739) antibody. Western blot analysis of extracts from NIH/3T3 cells, using SP1 (Phospho-Thr739) antibody.