Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Histone H3.1 around the phosphorylation site of serine 10.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P68431
Applications:
WB: 1:500~1:1000 ELISA: 1:20000
Specificity Sensitivity:
Histone H3.1 (Ab-10) antibody detects endogenous levels of total Histone H3.1 protein.
References:
Dai J, et al. (2005) Genes Dev 19(4): 472-488.
Yih LH, et al. (2005) Carcinogenesis 26(1): 53-63.
Western blot analysis of extracts from COLO205 cells using Histone H3.1 (Ab-10) antibody (Line 1 and 2) and Histone H3.1 (phospho-Ser10) antibody (Line 3 and 4).