• Catalog Number:

  E1A7215A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SEK1/MKK4 around the phosphorylation site of serine 80 (T-H-SP-I-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P45985

• Applications:

  WB: 1:500~1:1000 IHC:1:50~1:100 ELISA: 1:5000

• Specificity Sensitivity:

  SEK1/MKK4 (phospho-Ser80) antibody detects endogenous levels of SEK1/MKK4 only when phosphorylated at serine 80.

• References:

  Park HS, et al. (2002) J Biol Chem; 277(4): 2573-8.

Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using SEK1/MKK4 (Phospho-Ser80) Antibody. Right: The same antibody preincubated with synthesized phosphopeptide.

Western blot analysis of extracts from NIH/3T3 cells using SEK1/MKK4 (phospho-Ser80) antibody.