• Catalog Number:

  E1A7164B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human NF-κB p100/p52 around the phosphorylation site of Serine 865.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q00653

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 IP: Various Dilution

• Specificity Sensitivity:

  NF-κB p100/p52 (Ab-865) Antibody detects endogenous levels of NF-κB p100/p52 protein around Serine 865.

• References:

  Qu Z, et al. (2004) J Biol Chem. 279(43): 44563-44572. Xiao G, et al. (2001) Mol Cell. 7(2): 401-409. Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20. Haskill S, et al. (1991) Cell 65:1281-1289.

Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using NF-κB p100/p52 (Ab-865) Antibody; Right: The same antibody preincubated with synthesized peptide.

Western blot analysis of extracts from ovary cancer cells. Line1: Using NF-κB p100/p52 (Ab-865) Antibody; Line2: The same antibody preincubated with synthesized peptide.