• Catalog Number:

  E1A7207A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Raf1 around the phosphorylation site of serine 338 (R-D-SP-S-Y).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P04049

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Alter Name:

  C-RAF, C-Raf, CRAF, Protein kinase raf 1, RAF proto-oncogene serine/threonine-protein kinase, RAF-1, RAF1, Raf proto-oncogene serine/threonine-protein kinase, Raf-1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, kinase Raf1

• Specificity Sensitivity:

  Raf1 (phospho-Ser338) antibody detects endogenous levels of Raf1 only when phosphorylated at serine 338.

• References:

  Wolf Oehrl, J. Biol. Chem., May 2003; 278: 17819 - 17826. Deanna G. Adams, J. Biol. Chem., Dec 2005; 280: 42644 - 42654. Michael A. Beazely, Mol. Pharmacol., Jan 2005; 67: 250 - 259. Dwayne L. Barber, Blood, Jan 1997; 89: 55 - 64.

• All Sites:

  Human: Ser338; Mouse: Ser338; Rat: Ser338

Western blot analysis of extracts from NIH-3T3 cells treated with PMA (125ng/ml, 15mins), using, using Raf1 (phospho-Ser338) antibody (Line 1 and 2).