• Catalog Number:

  E1A1075A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IREB1 around the phosphorylation site of serine 711 (Y-G-SP-R-R).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P21399

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  ACO1, Aconitate hydratase, EC 4.2.1.3, Ferritin repressor protein, IRE-BP 1, IRE1, IREBP1, IRP1, aconitase, citrate hydro-lyase, iron regulatory protein 1, iron-responsive element binding protein 1

• Specificity Sensitivity:

  IREB1 (Phospho-Ser711) antibody detects endogenous levels of IREB1 only when phosphorylated at serine 711.

• References:

  Hirling H., Nucleic Acids Res. 20:33-39(1992). Rouault T.A., Proc. Natl. Acad. Sci. U.S.A. 87:7958-7962(1990). Hentze M.W., Nucleic Acids Res. 19:1739-1740(1991).

• All Sites:

  Human: Ser711; Mouse: Ser711; Rat: Ser711

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using IREB1 (Phospho-Ser711) antibody. Western blot analysis of extracts from 293 cells, treated with insulin (0.01U/ml, 30mins), using IREB1 (Phospho-Ser711) antibody.