• Catalog Number:

  E1A7198A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKR around the phosphorylation site of threonine 446 (K-R-TP-R-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P19525

• Applications:

  IHC: 1:50~1:100 ELISA: 1:10000

• Alter Name:

  EIF2AK2, Interferon-induced double-stranded RNA-activated protein kinase

• Specificity Sensitivity:

  PKR (phospho-Thr446) antibody detects endogenous levels of PKR only when phosphorylated at threonine 446.

• References:

  Abujiang Pataer, et,al. (2002) Cancer Res ; 62: 2239. K. D. Ryman, et,al. (2005) J. Virol ; 79: 1487-1499. Susana Guerra, et,al. (2006) J. Biol. Chem ; 281: 18734-18745.

• All Sites:

  Human: Thr446; Mouse: Thr409

Western blot analysis of extracts from K562 cells untreated or treated with Starvation (24 hours), using PKR (Ab-446) antibody (Line 1 and 2) and PKR (phospho-Thr446) antibody (Line 3 and 4).