• Catalog Number:

  E1A7074A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p44/42 MAP Kinase around the phosphorylation site of tyrosine 204 (T-E-YP-V-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P27361

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Alter Name:

  Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAPK 1, p44-ERK1, ERT2, p44-MAPK, Microtubule-associated protein 2 kinase

• Specificity Sensitivity:

  p44/42 MAP Kinase (Phospho-Tyr204) antibody detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at tyrosine 204.

• References:

  Jun Tan, et al. (2000) J. Biol. Chem; 275: 37224. Tete Hannken, et al. (2000) J. Am. Soc. Nephrol; 11: 1387. Jun Tan, et al. (1999) J. Immunol; 163: 6614. Reinoud Gosens, et al. (2006) Am J Physiol Lung Cell Mol Physiol; 291: L523-L534.

• All Sites:

  Human: Tyr204; Mouse: Tyr204; Rat:Tyr204

Western blot analysis of extracts from Jurkat cells, untreated or PMA-treated (200 ng/ml, 15mins), using p44/42 MAP Kinase (Ab-204) antibody (Line 1 and 2) and p44/42 MAP Kinase (Phospho-Tyr204) antibody (Line 3 and 4).